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Assays List

We provide in vitro services with in house capabilities with Absorbance or Fluorescence or Luminescence based high-throughput (1536 well) assays using primary human cells or cell lines either in  2D or 3D  co-culture format.

Cell Types

Healthy donor derived cells: Primary human cells: skin epidermal-progenitor keratinocytes, melanocytes, dermal – Fibroblasts, papilla cells, Mast cells, dermal, Gingival/Oral-Epithelial & Fibroblast cells, Corneal epithelial cells, mesenchymal stem cells from adipose, Wharton Jelly, blood, skin and bone marrow, Tenocytes from Patellar or Achellies, PBMC, Subsets of T and B cell lymphocytes and other cells that are commercially available.

Immortalized cell lines: any cell lines of your interest that are commercially available 

Almost all the cells are cultivated using our interference-free cell culture media (own IP formulations)

Note: Cell lines are subject to license fee from providers like ATCC or DSMZ or ECACC for commercial usage excluding cell lines purchase price. Usage of cell lines are also subject to notify to local regulatory authorities and approval.

Assays

Function Assay Technology
Cell Health - Viability/Proliferation/cytotoxicity
MTT, MTS, WST-8, PrestoBlue, Alamar blue, LDH, ATP and other markers
Absorbance, Fluorescence, Luminescence
Inflammation
Cytokines
Absorbance, QPCRs
Allergy
Histamine, Tryptase, PAF/Lipid, Proteases, Cytokines-chemokines, Nitric oxide, Endothelin
Absorbance, Fluorescence, Luminescence
Oxidative stress (ROS, DNA/RNA damage, lipid peroxidation, and protein oxidation/nitration)
DCF-DA -live cell 8-OHdG, Lipid hydroperoxide, Lipid peroxidation, Protein carbonyl content, DNA damage – apurinic/apyrimidinic sites
Anti-oxidant capacity
Total antioxidant capacity (copper and Ferric based)

Ascorbic acid, NAD/NADH, NADP/NADPH, GSH/GSSG ratio, Thio

Intracellular glutathione (GSH)

GST
Superoxide dismutase
Glutathione reductase
Xanthine oxidase
Glutathione peroxidase
Aconitase
Catalase
Thioredoxin reductase
NQO1
Peroxidase
Absorbance, Fluorescence
Apoptosis/Necrosis
JC-1 mitochondrial transmembrane potential, caspase or non-caspase or caspase- substrase based
Absorbance, Fluorescence, Luminescence
Hypoxia
In-Cell ELISA for Hif1a + BNIP3
Absorbance
Enzyme assays related to diseases or ageing
Tyrosinase, β-galactosidase, N-acetylglucosaminidase, deacetylases, PARP, methyltransferases, dipeptidyl peptidases, lipid kinases, tyrosine phosphatases and more
Absorbance, Fluorescence
Metabolism
Glucose, Lactate, Amylase, Glycogen, Pyruvate,Creatine, Glutamate, ATP, ADP/ATP, Phosphate, Pyrophosphate, NADH, NAD, NADHP, NADPH and several others
Absorbance, Fluorescence, Luminescence
Signaling mechanisms
SiRNA or pathway inhibitors
SYBR or multiplex QPCRs or protein expression
In Vitro Drug toxicity and metabolism (hepatology)

Example using HepaRG cells
Screening, toxicity, drug metabolic enzyme inhibition or activation
Absorbance, Fluorescence, Luminescence
Cell or tissue localization
Antigens of choice
Immunohistochemistry, Immunocytochemistry, Immunofluorescence

General Technologies

Gene expression
QPCRs
SYBR® Green and Probe-based (multiplex possible)
Protein expression
ELISA
Absorbance, alpha ELISA, (multiplexing possible)

For proteomics and metabolomics, please contact us. We have quick solutions ready to suite your needs.