Assays List
We provide in vitro services with in house capabilities with Absorbance or Fluorescence or Luminescence based high-throughput (1536 well) assays using primary human cells or cell lines either in 2D or 3D co-culture format.
Cell Types
Healthy donor derived Primary human cells: skin epidermal – progenitor keratinocytes, melanocytes, dermal – Fibroblasts, papilla cells, Mast cells, Gingival/Oral-Epithelial & Fibroblast cells, Corneal epithelial cells, Mesenchymal Stem cells from adipose, wharton Jelly, blood and bone marrow, Tenocytes from Patellar or Achellies, PBMCs, Subsets of T and B cell lymphocytes.
Immortalized cell lines: any cell lines of your interest that are commercially available
Almost all the cells are cultivated using our interference-free cell culture media (own IP formulations)
Note: Cell lines are subject to license fee from providers like ATCC or DSMZ or ECACC for commercial usage excluding cell lines purchase price. Usage of some cell lines are also subject to notify to local regulatory authorities and approval.
Assays
| Function | Assay | Technology | |
| Cell Health – Viability/Proliferation/cytotoxicity | MTT, MTS, WST-8, PrestoBlue, Alamar blue, LDH, ATP and other markers | Absorbance, Fluorescence, Luminescence | |
| Inflammation | Cytokines | Absorbance, QPCRs | |
| Allergy | Histamine, Tryptase, PAF/Lipid, Proteases, Cytokines-chemokines, Nitric oxide, Endothelin | Absorbance, Fluorescence, Luminescence | |
| Oxidative stress (ROS, DNA/RNA damage, lipid peroxidation, and protein oxidation/nitration) | DCF-DA -live cell 8-OHdG, Lipid hydroperoxide, Lipid peroxidation, Protein carbonyl content, DNA damage‚ apurinic/apyrimidinic sites | Absorbance, Fluorescence | |
| Anti-oxidant capacity | Total antioxidant capacity (copper and Ferric based) Ascorbic acid, NAD/NADH, NADP/NADPH, GSH/GSSG ratio, Thio Intracellular glutathione (GSH) GST Superoxide dismutase Glutathione reductase Xanthine oxidase Glutathione peroxidase Aconitase Catalase Thioredoxin reductase NQO1 Peroxidase | Absorbance, Fluorescence | |
| Apoptosis/Necrosis | JC-1 mitochondrial transmembrane potential, caspase or non-caspase or caspase- substrase based | Absorbance, Fluorescence, Luminescence | |
| Hypoxia | In-Cell ELISA for Hif-1a + BNIP3 | Absorbance | |
| Enzyme assays related to diseases or ageing | Tyrosinase, beta-galactosidase, N-acetylglucosaminidase, deacetylases, PARP, methyltransferases, dipeptidyl peptidases, lipid kinases, tyrosine phosphatases and more | Absorbance, Fluorescence | |
| Metabolism | Glucose, Lactate, Amylase, Glycogen, Pyruvate,Creatine, Glutamate, ATP, ADP/ATP, Phosphate, Pyrophosphate, NADH, NAD, NADHP, NADPH and several others | Absorbance, Fluorescence, Luminescence | |
| Signaling mechanisms | SiRNA or pathway inhibitors | SYBR or multiplex QPCRs or protein expression | |
| In Vitro Drug toxicity and metabolism (hepatology) Example using HepaRG cells | Screening, toxicity, drug metabolic enzyme inhibition or activation | Absorbance, Fluorescence, Luminescence | |
| Cell or tissue localization | Antigens of choice | Immunohistochemistry, Immunocytochemistry, Immunofluorescence | |
General Technologies
| Gene Expression | QPCRs | SYBR® Green and Probe-based (multiplex possible) |
| Protein expression | ELISA | Absorbance, alpha ELISA |
For proteomics and metabolomics, please contact us. We have quick solutions ready to suite your needs.
Assays
| Function | Assay | Technology |
|---|---|---|
|
Cell Health - Viability/Proliferation/cytotoxicity |
MTT, MTS, WST-8, PrestoBlue, Alamar blue, LDH, ATP and other markers |
Absorbance, Fluorescence, Luminescence |
|
Inflammation |
Cytokines |
Absorbance, QPCRs
|
|
Allergy |
Histamine, Tryptase, PAF/Lipid, Proteases, Cytokines-chemokines, Nitric oxide, Endothelin |
Absorbance, Fluorescence, Luminescence
|
|
Oxidative stress (ROS, DNA/RNA damage, lipid peroxidation, and protein oxidation/nitration) |
DCF-DA -live cell
8-OHdG, Lipid hydroperoxide, Lipid peroxidation, Protein carbonyl content, DNA damage – apurinic/apyrimidinic sites |
|
|
Anti-oxidant capacity |
Total antioxidant capacity (copper and Ferric based) Ascorbic acid, NAD/NADH, NADP/NADPH, GSH/GSSG ratio, Thio Intracellular glutathione (GSH) GST Superoxide dismutase Glutathione reductase Xanthine oxidase Glutathione peroxidase Aconitase Catalase Thioredoxin reductase NQO1 Peroxidase |
Absorbance, Fluorescence |
|
Apoptosis/Necrosis |
JC-1 mitochondrial transmembrane potential, caspase or non-caspase or caspase- substrase based |
Absorbance, Fluorescence, Luminescence
|
|
Hypoxia |
In-Cell ELISA for Hif1a + BNIP3 |
Absorbance |
|
Enzyme assays related to diseases or ageing |
Tyrosinase, β-galactosidase, N-acetylglucosaminidase, deacetylases, PARP, methyltransferases, dipeptidyl peptidases, lipid kinases, tyrosine phosphatases and more |
Absorbance, Fluorescence
|
|
Metabolism |
Glucose, Lactate, Amylase, Glycogen, Pyruvate,Creatine, Glutamate, ATP, ADP/ATP, Phosphate, Pyrophosphate, NADH, NAD, NADHP, NADPH and several others |
Absorbance, Fluorescence, Luminescence
|
|
Signaling mechanisms |
SiRNA or pathway inhibitors |
SYBR or multiplex QPCRs or protein expression
|
|
In Vitro Drug toxicity and metabolism (hepatology) Example using HepaRG cells |
Screening, toxicity, drug metabolic enzyme inhibition or activation |
Absorbance, Fluorescence, Luminescence |
|
Cell or tissue localization |
Antigens of choice |
Immunohistochemistry, Immunocytochemistry, Immunofluorescence |
General Technologies
|
Gene expression |
QPCRs |
SYBR® Green and Probe-based (multiplex possible)
|
|
Protein expression |
ELISA |
Absorbance, alpha ELISA, (multiplexing possible) |
For proteomics and metabolomics, please contact us. We have quick solutions ready to suite your needs.
